The teams take off from their Pit Stop at the Fonte Aretusa in Sircusa, Italy and travel 350 miles by train and ferry to Rome, Italy where they search for a man on a yellow-and-red scooter. The teams then receive a clue directing them to travel a mile and search for horse carriages where they'll find black leather portfolios. These portfolios contain information that helps them to ""decode"" a clue related to the movie The DaVinci Code, resulting in a prize for one team. They then travel to Athens, Greece, 665 miles away and find a marketplace in Agora. One team surges ahead with a fast forward that requires them to break plates, while the others travel by 70 miles by train to Ishthmos Station. Some teams fall behind after a failed attempt at the Fast Forward and others make a critical mistake by taking a bus part of the way instead of the train. The Roadblock requires one member of each team to to bungee jump over 200 feet into the Corinth Canal. It's immediately followed by a Detour --- Herculean Effort (a set of Greek games --- discus toss, javelin, and wrestling) or It's All Greek to Me (search an archaelogical site for Greek letters and get help from locals in translating to find the name of a location.) Finally, the teams drive 107 miles to the Pit Stop at the Fortress of Rion, where two teams face penalties for their earlier error and another team is eliminated.

• Elise Doganieri
• Bertram van Munster
• Jerry Bruckheimer

• Kim DeJesus
• Phil Keoghan
• Cody Nickson